EUROMEDICA  Hanover 1-2  Juni 2007	Advanced methods of diagnosis, treatment and prophylactics
	European Academy of Natural Sciences, Hanover European Scientific Society, Hanover Russian Academy of Natural Sciences, Moscow

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Functioning of the cardio-vascular system is disturbed by oxidative stress. Reactive oxygen species (ROS) are involved in regulation of activity of cardiomyocytes, smooth muscle cells and endothelium of blood vessels under normal physiological conditions. Abundant levels of ROS produced by the blood and vessel cells accompany heart and vascular diseases being an additional factor of damage. The capacity to catch and neutralize free radicals of oxygen is the characteristic feature of the natural compounds containing in various plants. The combination of natural components in the “Capinorm” phytopreparation provides the most favorable effect on veins and capillaries. The phytoremedy developed by the Scientific Production Association “MEDICOMED” (Moscow, Russia) includes standardized dry plant extracts: from the grass of Bupieurum multinerve DC; from the flowers of Hibiscus sabdariffa (L.); from the one year’s foliaceous shoots of Pentaphylloides fructosa (L.) O. Schwarz), and dihydroquercetin - complex of bioflavonoid.

The purpose of this work was to study antioxidant properties of the above mentioned composition. The work was carried out using in vitro models including peripheral blood of the health donor-volunteers, isolated granulocytes and cell-free enzyme reaction. A level of ROS was estimated by the method of chemiluminescence analysis with the use of the chemiluminometre “CHEMILUM-12” designed at the Institute of Cell Biophysics of the Russian Academy of Sciences (Russia). It was revealed that the phytoremedy “Capinorm” decreased level of the activated ROS production in the whole human blood in dose-dependent manner. Addition of 0.0005% solution of the preparation inhibited the blood cells response on 0.5 mg/ml opsonized zymosan by 50%. Neutrophils produce ROS more actively then other cells in blood, so the remedy was tested in different dilutions (from 10³ to 10⁶ times) on the isolated cells. Pretreatment of the cells with the Capinorm significantly inhibited intensity of the neutrophil respiratory burst, induced by chemotactic peptide N-formil-Met-Leu-Phe (fMLF). FMLF is the product of bacteria degradation, which presents in very high concentration in inflammatory center and forms a chemotactic gradient in tissues. Effect of the preparation depended on a dose: significant effect was revealed in concentration closed to 0.000005%. The preparation in high concentrations suppressed completely the activity of NADPH oxidase, the transmembrane enzyme complex crucial for the ROS production in phagocytes, neutrophils including. It is possible that the Capinorm preparation can influence an activity of other enzymes of the NOX family in the vessel cells, preventing from excessive ROS production. The property of the remedy as potential “trap” for ROS was tested on the cell-free enzyme reaction “xanthine – xanthine oxidase”. Superoxide-anion is main product of this reaction. Its reduction gives rise other different oxygen intermediates in chain reactions. Dose-dependent inhibition of superoxide anion generation was shown correlated with effect of superoxide dismutase known as antioxidative enzyme in the organism. Significant effect was obtained in addition of the remedy in the 0.000005% concentration. The preparation diluted 10²-10³ times depressed the reaction completely.

The data obtained indicate that the phytoremedy “Capinorm” has an antioxidant activity in the whole blood, in the isolated cells and in the enzyme reaction. “Capinorm” demonstrates high activity directed to lower a level of ROS. It acts in dilution as high as 100000 times and influences to decrease activity of the enzymes produced ROS and to catch superoxide-anion radical being an initial form of the extremely reactive intermediates of oxygen, nitrogen and chlorine. The properties of the remedy as antioxidant provided by revealed mechanisms would be favorable to support functional state of cardio-vascular system.