EUROMEDICA  Hanover 6-7  Juni 2008	Advanced methods of diagnosis, treatment and prophylactics
	European Academy of Natural Sciences, Hanover European Scientific Society, Hanover Russian Academy of Natural Sciences, Moscow

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Now in modern medicine there is a tendency to use natural medicines. One of groups of natural medical products is essence. Essential oils are flying, mobile liquids of a complex organic structure. Essential oils have a various color and a characteristic smell. They are soluble in spirit, ether, fats, and a little soluble in water. Essential oils of Artemisia pontica and an Artemisia jacutica contains α-pinen, camphor, isobutyric acid, ethers of butyric acid and formic acid, phenols, aquiline. The basic operating substance of this essence is chamazulene. Chamazulene concentration in the essential oil of Artemisia pontica is 13 %, and 40 % in the essential oil of Artemisia jacutica. Chamazulene possesses complex antiphlogistic, anesthetic, anti-edematous activities. Essential oils received by a method of steam distillation from a grass collected in a flowering phase. Essential oils of Artemisia pontica and a Artemisia jacutica is a complex of operating substances and their use is especially effective for treatment of various wounds since they possess anti-inflammatory, bacteriostatic, wound healing activity, and they render clearing action. At treatment of burns by these essential oils blisters, post burn hems and maculas are not formed.

In this connection the purpose of our research work was development of a soft drug formulation on the basis of these essential oils. 8 ointment of various compositions bases were selected for investigation. Ointments contained 3 % of essential oil. Determination of bioavailability of essential oil spent using a method of direct diffusion to an agar. It was established that ointments on the basis of polyethylene glycol have the best bioavailability. The ointment base has the following composition: Polyethylene glycol-1500: Polyethylene glycol400: Twin-80 (4, 9:4, 9:0, 25). Ointments on the basis of polyethylene glycol are ointments emulsive type, they have good organoleptic properties and one well put on a skin surface.

Wound healing activity of ointment determined on mice by degree of rescuing a tail after an afflicted thermal burn. Tails of animals were put into hot water (55°C) for 30 seconds. Ointments applied on tails of animals daily. The experiment was conducted during 25 days. Ointment with essential oil of Artemisia jacutica have the greatest wound healing activity, at its application the percent of safety of a tail is 55,2 %, it is higher than in control group (26,48%) and comparison group (36,46 %). Then we conducted histologic research of fabrics of tails of animals. For histologic research, by means of the sliding microtome did microscopic sections, thickness of 5-6 micron from two sites of a tail (the beginning of a tail and the tail end). Decalcification of fabrics was carried out, and the material was painted hematoxylin and eosin. In control group the inflammation was shown seepage fabric by leukocytes, a hypostasis of fabric, plethora, and stagnation of blood in vessels. In fabric of tails of the animals that were treated with the experimental ointment, inflammatory reaction was absent, the sclerosis and regeneration of epithelium was observed. Thus, the studied ointment positively influences all stages of the inflammation and accelerates healing of wound.