EUROMEDICA  Hanover 6-7  Juni 2008	Advanced methods of diagnosis, treatment and prophylactics
	European Academy of Natural Sciences, Hanover European Scientific Society, Hanover Russian Academy of Natural Sciences, Moscow

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Sulphur derivatives of the spatially-difficult phenols present great interest among water-soluble antioxidants, characteristic feature of which is their expressed antioxidant activity. Ditretiophenyl (3-(3, 5 di-tret-butyl-4-hydr ophenyl)propyltiosulphonat sodium) is crystal powder of white colour, with a specific smell and a bitterish taste. In the Uf-spectrum there is one maximum of absorption at 277 nm. Ditretiophenyl possesses hydro properties: it is easily dissolve in water, dissolve in spirits and very little in chloroform and benzene.

Ditretiophenyl in water solutions under the influence of acids and the bases form at once a disulphide, or through a stage of formation in the intermediate product – tiole, on the hydrolysis process influence: percentage of acid in the reactionary environment, temperature and duration of reaction.

The critical toxicology of ditretiophenyl in peritoneum by mice is 175mg/kg (LD50). On the model of Cu²⁺ - induction of oxidation metyloleate at 60°C in water - emulsion to environment (calculate the induction period), and on the model Cu²⁺ - (5MKM CuSO₄) and Fe²⁺- induction (25MKM FeSO₄) oxidation of LNP at 37°C in a current of 30 minute (by a fluorescent method measured accumulation reacting with thiobarbiturat acid of products), the expressed antioxidant activity ditretiophenyl is established in comparison with structural analogues and known antioxidants: ionol and phenozan potassium.

A research on immunotropic properties of the ditrethiofenyl was carried out in two dose 20 mgk/ml and 200 mgk/ml for the spontaneous and ConA, PWM, LPS mitogen - stimulated proliferation of the cells of spleen in the culture in vitro. It is discovered, that the ditrethiofenyl dose-dependent suppress the proliferation of immunocompetent cells by over 70%. The result of experiments in vivo on studying of as much as possible transferable dose ditretiophenyl (35 mg/kg) in peritoneum introduction during 7 days at mice showed, that ditrethiofenyl possesses an immunodepressive activity, the lower level nucleus contain cells of peripheral blood more than on 20 %. Ditretiophenyl in a dose of 10 mg/kg the influence on primary gymoral the immune answer (IgM) in vivo - by absolute and relative quantity antibody cells in a spleen, show authentic results in absolute quantity IgM on 40 % and 29 %, and relative quantity IgM on 27 % and 23 %. These immunodepression activities have been tested on experimental model immunocomplex gromerynephrit. Ditretiophenyl in a dose of 35mg/kg in peritoneum every other day authentically reduces cardinal number the albumen into the urine that testifies to an anti-inflammatory activity.

Research radioprotection activity are shown that at low concentration ditrethiofenyl in doses of 2 mg/kg and 10 mg/kg entered in peritoneum in volume of 0,5 ml daily in a current of four days, insignificantly increases the life expectancy of mice after a lethal irradiation (in a dose 850R), however not exceeding maximal of 10 days. At increase in a dose of 20 mg/kg the proof rise to 90% of survival rate of the animals to a sharp lethal irradiation is observed.

Thus, ditrethiofenyl the water-soluble compounds, showing in experimental models in vitro and in vivo expressed antioxidant, anti-inflammatory and radioprotection activities, can find application as a nontoxic immunodepressant.