EUROMEDICA  Hanover 6-7  Juni 2008	Advanced methods of diagnosis, treatment and prophylactics
	European Academy of Natural Sciences, Hanover European Scientific Society, Hanover Russian Academy of Natural Sciences, Moscow

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Probiotic strain of L. delbrueskii singled out from a feces of healthy children was deposited in the collection as a republican perspective for the development of probiotic ferment in dairy industry. L. delbrueskii has a high antagonistic activity to Escherichia coli, Serratia marcescens, Klebsiella ozaenae, Streptococcus pyogenes, Staphylococcus aureus, Salmonella typhimurium, Candida albicans, Proteus mirabilis, resistance to Amoxiclav, Furazolidone, Ampicillin, Cephazolin, Vancomycin, Kanamycin, Roxithromycin, with a titratable acidity 70° T. Activities of the probiotic strain during cultivation in the fermentation for 17 hours at 37° C, in MRS broth medium production “Himedia” amounted to 1*10⁷ CFU / ml.

In order to improve the economic efficiency of crop production, a study was conducted on the selection of an optimal growth medium with cheaper raw materials. The analysis showed that the most effective medium for the cultivation of L. delbrueskii is the environment containing whey, sodium citric acid, sodium phosphate, potassium hydrophosphate, magnesium sulfate, yeast extract, glucose and casein hydrolysate.

For study the culture was prepared by ten passages on the given medium, followed by fermentation for 17 hours at a temperature of 37° C, the amount of contributed inoculum was 5%, pH 6.0. The evaluation was carried out by limiting dilution and subsequent seeding on the medium MRS-4 and the definition of optical density at a spectrophotometer at a wavelength of 600 nm.

The analysis showed a high enzymatic activity of the strain L. delbrueskii during cultivation of an optimized environment for 1*10⁹ CFU / ml. In our opinion, this is due to the rich composition of the main raw material in a serum containing proteins, fats, calcium, magnesium, vitamins and lactose, which are important for crop growth factors for the cultures of genus Lactobacillus. Moreover, milk serum is a waste diary products, othervise a cheap raw material.

Application of an optimized nutrient product is a significant factor influencing the cost-effectiveness in the production of biological-based probiotic strain of L. delbrueskii, facilitating reduction of costs for raw materials in more than twenty times.