EUROMEDICA  Hanover 6-7  Juni 2008	Advanced methods of diagnosis, treatment and prophylactics
	European Academy of Natural Sciences, Hanover European Scientific Society, Hanover Russian Academy of Natural Sciences, Moscow

Home

Abstracts

Contact

Disclaimer

The purpose of our research was to learn the level of functional activity of ribosomal genes (FARG) among children with cerebral palsy (CP), their parents and to compare the obtained results with ones in control groups. Also we estimated polymorphisms of gene TP53 among children with CP and their parents.

Materials and methods. The material of our research consisted of 60 children with CP, 46 mothers and 17 fathers of children with CP. Control group consisted of 37 healthy children and 70 healthy adults of Kursk region.

Cultivation of cells and their colouring was performed according to conventional method. The level of FARG was estimated on metaphase chromosomes with the help of light microscopy by sight using the five-ball scale (Lapunova N.A., 2001).

DNA was extracted from the venous blood of children with CP for estimation of gene TP53 polymorphisms. Extracted DNA was put to PCR-test. After that restriction by BspFNI TP53 enzyme and electrophoresis of nuclear acids in agarose gel were performed with the following estimation of polymorphisms: P 199 (mutation), R 113+86 (wild). The score distribution was: R/R (wild) – 0 scores, P/R (heterozygous mutation) – 1 score, P/P (homozygous mutation) – 2 scores. The level of trustworthiness was taken at p<0,05.

Results and their discussion. It was determined that average index of FARG among children with CP was 18,4±0,24 conventional units (c.u.) which was statistically proved to be lower than in control group of children – 19,43±0,27 c.u. (t=3,77). There were discovered no statistically proved differences among patients by sex, average FARG among boys was 18,57±0,27 c.u. and among girls – 18,04±0,48 c.u. Neither we found any statistically proved differences between indices of FARG among children with CP and their parents. Average indices of FARG among fathers and mothers of children with CP didn’t differ much and were 18,07±0,38 c.u. and 18,73±0,23 c.u. correspondingly. However there were found statistically proved differences between indices of FARG among parents of children with CP and adults in control group.

There were found statistically proved differences in FARG between children with spastic hemiplegia, lower spastic diplegia and the group of healthy children: t=3,3 and t=2,59 correspondingly.

The estimation of polymorphisms of gene TP53 was performed using the score-system. The average quantity of score (s.) among children with CP was 0,62±0,09 s. There were found no statistically proved differences among patients by sex. The average quantity of scores among fathers and mothers of children with CP was 0,53±0,12 s. and 0,72±0,09 s. correspondingly and didn’t statistically differ from each other. There were neither any statistically proved differences in scores between children with CP and their parents.

Study of gene TP53 polymorphisms among children with different forms of CP in comparison with each other and with their parents showed no statistically proved differences. As a result of correlation analysis, poor correlation link was revealed between FARG and sex; It was confirmed with literature data. The same poor link was revealed between FARG and polymorphisms of gene TP53. Thus our research proves the presence of hereditary-genetic component such as FARG and polymorphisms of gene TP53 in the origin of CP. Consequently this shows the new direction in the solving the problem of

treating and early prognosing of CP. However, gathered results in the research of gene TP53 polymorphisms only do not provide complete information about the role of hereditary-genetic component in the structure of CP morbidity. This requires further research of polymorphisms of other genes.