EUROMEDICA  Hanover 3-4  Juni 2010	Advanced methods of diagnosis, treatment and prophylactics
	European Academy of Natural Sciences, Hanover European Scientific Society, Hanover Berliner Medizinischen Gesellschaft, Berlin

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The experimental estimation of biological efficiency of the samples of potable water «AQUA FORTE» (TEMPO) was carried out. The samples are received by means of low-temperature activation of the tap water by original technique of The Company “Cryonord”. Morphological, functional, biochemical and analytical methods are used in the work.

Rats-males of Wistar line with initial body weight 180-200g and the age of 3-4 months were given water «AQUA FORTE» (TEMPO) within 2 months. Control group of animals were given initial tap water. The peripheral blue blood and the central lymph (a lifetime taking) and also thymus, lymph nodes and mesentery of a small intestine were chosen as objects of research. received after eutanasia of the animals under Ether narcosis. Morphometric and cytologic research was conducted by methods of light microscopy after histologic processing of the taken material. Revealing of the must cells and microvessels in the mesentery of small intestine was carried out after colouring according to Romanovsky-Gimza by means of light microscopy.

Ratio and distribution according to share contributions of the basic substrata of lipid peroxidation – the free fat acids – was studied by the method of highly effective capillary gas-liquid chromatography in the tests of the central lymph and blood whey. The free fat acids are capable to act as markers of development of the oxidizing stress leading to infringement of integrity of cellular membranes.

Biochemical researches of the blood whey were carried out for definition of general protein, albumin, urea, creatinin, cholesterol, triglycerides, β-lipoproteids, activity of enzymes AST and ALT. Lifetime flowmetry research of a peripheral capillary blood-groove in a small pillow of foot of the left finiteness of an animal was carried out by means of the serial laser analyzer of a capillary blood-groove.Analysis of profiles of the maintenance of microelements in the tests of blood and the central lymph was conducted by the method of atom-issue spectrometry with arc excitation of spectra.

Conclusions:

1. Specific biological effects of potable water «AQUA FORTE» (TEMPO) are revealed:
• Gentle metabolic changes in an organism of experimental animals: shift of the parameters, characterising albuminous and lipid metabolism, testifying to decrease in toxic loading on an organism of animals;
• Transformations of the structural organisation of the central body of immunity – thymus (morphometric and cytologic research), testifying to activation of lymphoproliferative function, activation of the cellular immunity;
• Activation of drainage-detoxication function of the mesentery lymph nodes: biological processing of a flowing lymph (barrier-filtration function) and acceleration of xenobiotics removing from an organism;
• Reduction of expressiveness of macrophage reaction in the structural elements of lymph nodes that testifies to decrease in toxic loading on an organism.
• Blood-groove activation, due to formation of its optimum regulation through must cells device, (change of the structural organisation of hemomicrocirculatory channels, parities of forms of the must cells).
2. Potable water «AQUA FORTE» (TEMPO) causes characteristic changes, comparable on intensity of appearance:
• Activation of lymphopoesis in the thymus;
• Activation of drainage-detixication function of the lymph nodes;
• Soft shifts in metabolism of the proteins and lipids.
3. Biosafety of potable water «AQUA FORTE» (TEMPO) is revealed; use of “thawed” water:
• Does not cause significant changes in the microelement status of an organism;
• The offered drinking mode does not render damaging action on permeability of cellular membranes, but shows the tendency to stabilization of antioxidant balance;
• Does not cause sharp changes of a peripheral blood-groove, but thus strengthens functional activity of microcirculatory vessels.

The regular use of potable water «AQUA FORTE» (TEMPO) promotes stimulation of protective forces of an organism, blood- and lymph-detoxication of the internal environment.