EUROMEDICA  Hanover 3-4  Juni 2010	Advanced methods of diagnosis, treatment and prophylactics
	European Academy of Natural Sciences, Hanover European Scientific Society, Hanover Berliner Medizinischen Gesellschaft, Berlin

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Cadmium, as one of the toxicant of the exogenous genesis, causes a big range of functional afflictions in the liver by decreasing protein and some enzymes synthesis, which participate in toxicant metabolism. One of the crucial tests is an assessment of the level of ALT and AST as the indicators of tissues’ damage and which efflux to the blood flow under the massive cells’ distraction.

This investigation was taken on the white rats over 1, 7, 14, 21 days after exposure. The level of ALT of the control group was 0,14±0,02 mkkat/l and AST - 0,15±0,02 mmkat/l as well. After the chronic intoxication with CdCl₂ in dosage of 1,5 mg/kg the level of ALT increased by 39% during the 1st day comparing with the control data. On the 7th day ALT decreased by 4% relatively the 1st day results. By the 14th day, its level declined by 4,5% again relatively the 7th day data. By the 21st day ALT failed by 9.5% comparing with the 14th day results and was equal 0,19 mkkat/l. This latter indicator was higher that the control data by 36%. In the same time the indicator of AST over 1 day was twice higher than the control results. By the 7th day AST’s level decreased by 3% comparing with the 1st day data. By the 14h day the falling was additionally by 6%. By the 21st days the level of AST decreased by 11% again and equal 0,24 mmkat/l. And this latter result was superior the control data by 60%.

Exposure by Cadmium Chloride as 3 mg (double dose) caused more significant changes in liver transaminases in the blood. At the 1st day the level of ALT increased in 3,3-fold relatively to the control data. By the 7th day, The was decreasing of that level comparing with the data of the 1st day by 17%. By the 14th day, The investigating indicator diminished by 13% relatively to the 7th day data. By the 21st day, The level of ALT decreased additionally by 15% and equal 0,29 mmkat/l. This latter value was twice higher than the control data. At the higher dose of Cadmium AST level was more sensitive. At the 1st day, AST data were upper in 4-fold relatively to the control ones. By the 7th day, These figures decreased just by 2%. By the 14th day, AST failed additionally by the 18%. By the 21st day, these data declined by 12% relatively the results of 14th day and were equal 0,44 mkkat/l. The latter figure was 2,9 - fold higher the control data. So, The aminotranferases’ content in the blood flow are cadmium chloride dose dependent and the compensating decrease of their levels goes gradually. But even on the 21st day the point of the transaminases was much higher than the control data. The statistic analysis of the records showed that the Ratio of Dependency (r) of ALT from the dose was 0,97 with P=0.008, for AST this ratio (r) was 0,94 with P=0,016. Therefore, More cadmium amount is given, more aminotransferases’ content is increased. During the time, when animals were not fed by the toxicant there was slow decreasing of the transaminases’ level, but even by the 21st day these data were much higher than the control ones. So, the rising of aminotransferases’ content in blood flow of rats who were exposed to cadmium chloride is reliable indicator of Cadmium deteriorating effect.