EUROMEDICAHanover23-24 Mai 2011 |
Advanced methods of diagnosis,
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European Academy of Natural Sciences, HanoverEuropean Scientific Society, Hanover |
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| A. Khama-Murad N. Heydarian K. Akhanchikova |
CHANGES OF RHEOLOGICAL INDICATORS OF ERYTHROCYTES IN PATIENTS WITH ISCHEMIC STROKE |
| Physiology Institute named after I. P. Pavlov, Russian Academy of Science,
Saint-Petersburg, Russia Saint-Petersburg State University named after an academician I.P. Pavlov, Saint-Petersburg, Russia Leningrad State University named after A. S. Pushkin, Saint-Petersburg, Russia |
An ability of erythrocytes to deformation and aggregation plays a significant role in pathogenesis of an ischemic stroke. Numerous research of ischemic affections of vascular system show deterioration of rheological properties of blood and, particularly, of erythrocytes. With appearance of the newest methods of erythrocytes’ plastic properties research there also appeared a possibility to specify our conceptions about an intensity degree of hemodynamic disorders in the case of some diseases.
Material and methods
We observed 12 men, who had been in a rehabilitation period in a clinic after an ischemic stroke; a control group consisted of 15 healthy patients. We have examined main rheological determinants of erythrocytes by straight adequate methods in whole blood. Deformability was examined by diffracting osmoscany method, aggregative properties – by piezodynamic method in a microcuvette. The methods which were used let us estimate not only integral indicators of erythrocytes’ deformability and aggregation, but also assess sphericity (S/V ratio), internal viscosity of erythrocytes, aggregations’ solidity and the speed of their spontaneous formation in whole equalized blood.
Results of research
An Omyn point, being true to osmolality of acquisition by erythrocytes an isovolemic sphere, has a tendency to shift into hyperosmotic field in patients: (148±7) mOsm in healthy people against (154±18) mOsm in ill ones. Deformability index in the control is (0,642±0,058) rel. units, and in ill patients – (0,580±0,054) rel. units (p=0,013). Hemoglobin dehydration (O’ parameter), leading to increase of internal viscosity of an erythrocyte’s contents, and, consequently, contributing noted decrease of deformability with this disease, is not denoted. A value of the 1st minute, or deformability index, being true to Omyn, is (0,065±0,024) rel. units in healthy people, and in ill patients (0,180±0,063), p<0,05.
An increase of both minimal (Uo parameter) and maximum (Uq parameter) solidities of erythrocytes’ aggregates in a microcuvette is noticeable. A speed of spontaneous aggregation, estimated by steepness of a right side of an aggregogramm, is (0,056±19) C-1 among healthy people and among ill ones (0,134±35) C-1, p<0,001.
Discussion
Erythrocytes of the patients, who had ischemic stroke, have a lower deformability and S/V ratio, also low steadiness to a hypotonic lysis, that is they are more subject to destruction on busy parts of blood flow. Moreover, a deterioration of hemodynamics conditions among such patients at the cost of sharp increase of aggregation properties, which contributes blood viscosity increase. In conclusion of this research we should maintain that a hemodynamics condition in patients with ischemic stroke undergoes a serious tension as a result of decrease of a deformability of the main mass of oxygen carriers and dramatic increase of their aggregation properties. A possibility of both pre-capillary, as a result of aggregates’ durability decrease and post-capillary thrombosis because of dramatic growth of erythrocytes’ aggregation speed.
