EUROMEDICA  Hanover 23-24  Mai 2011	Advanced methods of diagnosis, treatment and prophylactics
	European Academy of Natural Sciences, Hanover European Scientific Society, Hanover

Home

Abstracts

Contact

Disclaimer

The work is aimed at investigating dynamics of cellular composition of various structurally functional zones of a superficial cervical lymph node at experimental implantation of the titanium nickelide.

Methods of experiment. Research was studied on 60 white rats of a Wistar. Animals have divided into 3 groups: the first group of animals was control; by an animal of the second group it has been generated an implantation bed without introduction of the titanium-nickel alloy; animals of the third group were exposed to implantation of the titanium-nickel alloy in area of an alveolar process of the lower jaw. Cells of lymphoid tissue investigated with the help of an ocular graticule. Quantity of cells calculated an area of 1849 square microns in various structurally functional zones of a lymph node (germinal center, paracortex, medullary cords and lymphatic (medullar) sinus. At calculation were considered lymphocytes, immunoblasts, macrophages, reticular cells, mature plasmocytes, eosinophils. Statistical processing has been spent with application of software packages SPSS 9.0.

Results. The implantation of titanium nickelide changes cellular composition in a lymph node. There is statistically a decrease of the maintenance small lymphocytes, blast cells, macrophages and eosinophils in a lymphoid nodule with the germinal center. There is a decrease the maintenance of averages lymphocytes, immunoblasts reticular cells, and an increase the number of macrophages and eosinophils in paracortex. The cell population is become normal to an end of experiment in the paracortex. There is in the experiment beginning an increase the maintenance of mature plasmocytes in medullar cords of a lymph node. The quantity of macrophages, reticular cells, mature plasmocytes and eosinophils increased to an end of experiment in medullar cords of a lymph node. There is a decrease the maintenance of small and average lymphocytes and an increase the maintenance of eosinophils and macrophages by 7 days of supervision in medullar sinus of a lymph node. The number small lymphocytes, immunoblasts and eosinophils increased to end of experiment in in medullar sinus of a lymph node.

For 7-30 days of implantation was observed the authentic increase in the maintenance macrophages in germinal center, paracortex and medullary sinus of a lymph node. These changes are the activation of phagocytal reaction. It is often observed at protective reactions of an organism (Jurina N.A. et al. 1976; Rusina A.K., 1978). For all zones of a lymph node number decrease lymphocytes is characteristic. Decrease of lymphocytes is connected with delay of migration of cages in a lymph node, especially in paracortex. Certainly, the local proliferation cells are of great importance (Hussar J.P., 1980, Nieuwenhuis P. et al., 1976). The basic way of receipt lymphocytes in a lymph node are postcapillary venules with high endothelium. Circulation of lymphocytes is regulated of interdigital cells and macrophages in a lymph node (Fossum S. et al., 1983; Drayson M. et al., 1984). There is an increase in number of averages lymphocytes in the conditions of implantation. The average lymphocyte is the transitive form between immunoblast and small lymphocyte (Fossum S. et al., 1995). The increase the maintenance of averages lymphocytes is a development basis of proliferation of lymphoid tissue (Vasilev N.V. et al., 1972). In the germinal center marked maintenance increase immunoblasts. At the same time their maintenance in paracortex and medullary cords authentically decreased. Apparently, there is an oppression of differentiation of cells. These results will be coordinated with data of other scientists (Borodin Y.I. et al., 1985). Number of plasmocytes is connected with intensity of humoral immunity in a lymph node. We have noted positive changes in population of cells in a lymph node for 60 and 120 days of experiment. The quantity of lymphoid cells does not differ from control in structural and functional zones of a lymph node.

Conclusion. Morphokinetics of cellular populations of structurally functional zones takes place to be differently directed. The number of cells depends on functional specialization of a zone in a lymph node and from period of implantation.