EUROMEDICA  Hanover 23-24  Mai 2011	Advanced methods of diagnosis, treatment and prophylactics
	European Academy of Natural Sciences, Hanover European Scientific Society, Hanover

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Pulmonary emphysema (PE) is one of the most common diseases with the absence of medical solutions could stop the progression of the extracellular matrix destruction and lung function decline. One of the hypotheses of the pathogenesis of PE suggests that it is based on the imbalance of injury/regeneration of alveolar structures. Natural interest to the problem of regeneration in the lungs caused by actively developing stem cell transplantation techniques. Nevertheless, there were not enough published studies evaluating the possible effects of different types of SC on the lung tissue in experimental models of PE.

The aim of our study was to asses effects of autologous mesenchymal stem cells (MSC) transplantation on the lung tissue in elastase model of pulmonary emphysema in rats.

Methods: Forty 3-months old Wistar rats were randomized into 4 groups. Control group (1 group) was injected intratracheally 0,4 ml of normal saline, other animals (2-4 groups) received one intratracheal injection of 20 units (U) porcine pancreatic elastase in 0,4 ml of saline. Next day (2 group) and 7 day (3 group) rats were intravenously injected 2000000 autologous MSCs in 0,5 ml of saline. 4 group was used as emphysema control. Animals were euthanized at the 21 –st day.

Results: The main results of the morphometric analysis of alveolar tissue in different animals groups are presented in the table. Table Morphometric characteristics of acini in control and experimental groups

	alveolar ductus width (µm)	alveolar index (alveolar width/alveolar depth ratio)
Group 1 (saline control)	(n=65) 43,2±2,0**	(n=59) 0,78±0,04*
Group 2	(n=60) 66,5±3,8*	(n=57) 0,71±0,04*
Group 3	(n=38) 60,8±4,3*	(n=41) 0,49±0,06**
Group 4 (emphysema control)	(n=76) 100,0±2,0	(n=73) 1,16±0,04

n – amount of the measured alveolar ducti and alveoli *p<0,01 compared with group 4

** p<0,001 compared with group 4

Conclusions: Our study confirmed the possibility of regenerative lung tissue effect of autologous MSCs intravenous injected in experimental rat models of PE. The next step should be conducted the clinical trials to evaluate the efficacy and safety of this technology in human emphysema.

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