EUROMEDICAHanover23-24 Mai 2011 |
Advanced methods of diagnosis,
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European Academy of Natural Sciences, HanoverEuropean Scientific Society, Hanover |
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| T.A. Astashova M.A. Yurov S.V. Morozov V.V. Astashov |
FREE FATTY ACIDS IN ESTIMATION OF ENDOTOXEMIA, OXIDATIVE STRESS DEVELOPMENT AND THEIR CORRECTION IN EXPERIMENTAL PROSTATE INJURY |
| Institute of Clinical and Experimental Lymphology, SB RAMS, Novosibirsk, Russia
Novosibirsk Institute of Organic Chemistry, of the name of N.N. Vorozhtsov, SB RAS, Novosibirsk, Russia |
The purpose of this work was to study the role of the lymphatic system in the mechanism of oxidative homeostasis in experimental venous stasis in the pelvis, aggravated by chronic alcohol intoxication, and at correction by the use of the natural complex “MaxiSil”. To assess the state of pro-antioxidant balance and the development of endotoxemia the substrates of lipid peroxidation, free fatty acids (FFA), were used. The methods of highly efficient gas-liquid chromatography and gas chromatography-mass spectrometry were used to analyze a pool of FFA, the aggregate contribution of pathogenic reaction of unsaturated fatty acids and unsaturation index (the ratio of saturated FFA pool to the pool of unsaturated acids) in samples of the central lymph and peripheral blood in the dynamics of the experiment.
The transition from the normal living conditions to the conditions of the model in the central lymph samples is accompanied by acute increase of 39.7% in the total proportion of unsaturated fatty acids with a decrease of 12% in the contribution of saturated acids. Index value of saturation in the samples of the central lymph in venous stasis in the pelvis with chronic alcohol intoxication is 62,8% below than normal values, indicating the development of oxidative stress and lymphotoxicosis.
In the blood samples 90% increase in the pool of unsaturated fatty acids is revealed under condition of the model compared with the norm value and, as a consequence, the endotoxemia development. However, identified for these conditions 45.7% growth of the contribution of saturated fatty acids resulted in significantly lower (by 24% of normal values) decrease in saturation index compared with the situation in the central lymph. Taken together, these data indicate the greatest development of oxidative stress at the amplification of prooxidant processes in the central lymph.
The food supplement “MaxiSil” is an additional source of polyunsaturated fatty acids, including omega-3, bioflavonoids, iron, manganese and calcium, contributing to prevention of pathological changes in lymphatic region, in its detoxification and drainage structures. The choice of “MaxiSil” in the model of endo (exo) toxicosis is caused by its composition (lyophilized sea urchin roe, Leuzea Carthamoidis, Sweetvetch Tea, roots of Calamus and Manchurian Aralia) with strong antioxidant effect. It is shown that the use of the complex “MaxiSil” for dietary correction of rats ration during experimental venous stasis in the pelvis, aggravated by chronic alcohol intoxication, pools values of saturated and pathogenic unsaturated fatty acids in the samples of the central lymph were closer to normal quantities, thus the value of the saturation index saturation was only 12.4% below normal. At the same time transformation of FFA pools in the blood samples revealed for the conditions of correction has led to the value of the saturation index 8% over normal
Regulatory role of the lymphatic system in the maintaining pro-antioxidant balance in the control of oxidative homeostasis provides implementation of the mechanism of biomodified lymph throw into the bloodstream and is expressed in the stabilization of the relative pool of reactive unsaturated fatty acids.
Natural complex “MaxiSil” at synergistic unity of essential nutrients from plants and animals is able to provide corrective metabolic lymphostimulation to restore disturbed functions in lymphatic region and maintaining oxidative homeostasis.
