EUROMEDICA  Hanover 23-24  Mai 2011	Advanced methods of diagnosis, treatment and prophylactics
	European Academy of Natural Sciences, Hanover European Scientific Society, Hanover

Home

Abstracts

Contact

Disclaimer

The role of herpes infection in gastroduodenal diseases remains to be defined.

The aim of the study was to evaluate the prevalence of herpes virus infection in children and adults with chronic gastritis.

Materials and methods: 103 children at age of 7–17 and 49 adults at age of 18–78 suffering from chronic gastritis were examined. The chronic gastritis was diagnosed by endoscopic and morphological examination. Biopsies were obtained from the mid antrum and mid corpus. Biopsies were placed in 10% formalin and routinely embedded in paraffin blocks, then cut and stained in each local centre. The stained slides were examined by pathologist using the updated Sydney classification. Virus antigens were determined by immunohistochemical methods. Polyclonal antibody to herpes simplex 1 and 2 type (Dako), monoclonal antibody to early antigen of cytomegalovirus and monoclonal antibody to antigen (latent membrane protein ) of EBV( Novocactra) were used as primary antibody. Peroxidase –labelled steptavedin biotin complex were used for detection of virus antigens on deparaffined sections. Peroxidase activiti was visualized with a standart diamino-benzidine reaction. Brown granules were founded in structures of gastroduodenal mucosa as a result of reaction.

Results: Virus antigens at 70% gastric biopsies from children and 65,3% from adults were founded (EBV: children-44,8%, adults-40%, р>0,05; herpes simplex: children-34,9%, adults-26,5%, р>0,05; cytomegalovirus: children-16,5%, adults-18,4%, р>0,05; 2 mixed infections: children-22,3%, adults-16,4%, р>0,05, 3 mixed infections: children-1,9%, adults-2%, р>0,05 ). Virus antigenes detected in both biopsies (antral and fundal) at all patients .

EBV antigens in the form of a monoinfection detected in 28,1% of children and 26,7% of adults (р>0,05), herpes simplex antigens in the form of a monoinfection detected in 6,7% of children and 14,3% of adults, (р<0,05), cytomegalovirus antigens in the form of a monoinfection not detected in children and at adult detected in 20,2 % of cases (р<0,05).

Herpes simplex antigens in a stomach mucous had mainly perineural localization. It was marked also diffuse colouring of gastric epiteliocyts cytoplasm. EBV antigens in a stomach mucous had mainly perivascular localization. Cytomegalovirus antigens detected mainly in glandular cells of gastric mucous, thus in glands quite often there were signs of huge metamorphosis.

At positive VEB antigenes children, more often, than at children without virus infections the expressed activity of a gastritis as in a mucous membrane of a body of a stomach (38 % and 6,6 %, p <0,05), and in antral department (38 % and 10 %, p <0,05) was marked. At adults with VEB antigenes in a gastric mucous, more often, than at patients without virus infections it was marked not only the expressed activity of a gastritis in a mucous membrane of a body (46,2 % and 6,3 %, p <0,05), and antral department of a stomach (46,2 % and 9,4 %, p <0,05), but less often detected weak activity in fundal mucous (15,6 % and 56,2 %, p <0,05) and antral departments (23,1 % and 50 %, p <0,05) also. Herpes simplex antigenes did not influence activity of a gastritis at children and at adults. At the cytomegalovirus antigens positive adult patients more often, than at adults without virus infections in antral gastric mucous high activity of a gastritis (28,6 % and 9,4 %, p <0,05) was marked.

Conclusions: 1. The association between virus infection and chronic gastritis in children and adult were demonstrated. There was no difference in overall herpes virus infection prevalence between children and adult.

1. Epstein-Barr viruses and cytomegaloviruses influence a degree of activity of a chronic gastritis both at children, and at adults.
2. Availability of the mixed infections requires search of methods of differential diagnostics with cross reaction of antigenes different a herpes-viruses and a мixt-infections.