Euromedica 2011 V.N. Gorchakov S.I. Anohin O.V. Gorchakova Yu.P. Kolmagorov macro-and microelements of kidney and lymphatic node in dynamics of renalinsufficiency"

V.N. Gorchakov S.I. Anohin O.V. Gorchakova Yu.P. Kolmagorov	MACRO-AND MICROELEMENTS OF KIDNEY AND LYMPHATIC NODE IN DYNAMICS OF RENAL INSUFFICIENCY
	Institute of Clinical and Experimental Lymphology of Siberian Branch of Russian Academy of Medical Sciences, Novosibirsk, Russia Institute of Geology, Geophysics and Mineralogy of Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia Siberian District Medical Center of Medical and Biological Agency, Novosibirsk, Russia Novosibirsk State University, Novosibirsk, Russia

Necessity of study of microelement exchange in nephrology is dependent on participation of kidney and its regional lymphatic node in maintenance of homeostasis. Roentgen ﬂuorescent analysis with synchrotron irradiation (Institute of Nuclear Physics of Russian Academy of Sciences) and kidney and lymphatic node as a bio substrates have been used in study. Glycerol model with rats has been used for simulation of acute renal insufﬁciency (Borisova I.V. et al., 2003). It has been revealed that concentration of macro- and microelements in kidney differs from its concentration in lymphatic node. Concentrations of zinc (1,3 times), copper (1,5 times), iron and calcium (1,6 times) and selenium (1,6 times) are more in regional lymphatic node, than in kidney at the physiologic conditions. Concentration of potassium, manganese, chlorine and lead are almost the same in these organs per unit of mass. The mean concentrations of studied macro- and microelements in tissue of kidney and in tissue of lymphatic node in order of decreasing are the same K>Cl>Ca>Fe>Zn>Cu>Mn>Se>Pb.

At the 3-rd day of the acute renal insufﬁciency concentration of chlorine (1,8 times), iron (1,4 times), copper (1,4 times), zinc and selenium (1,2 times) are increased in tissue of kidney. Simultaneously concentration of manganese (1,3 times) is decreased. Concentrations of the other elements (calcium, lead, potassium) aren’t changed in comparison with control. At the 7-th day of acute renal insufﬁciency concentrations of the most macro- and microelements return to control values except chlorine, lead and iron, concentrations of them are increased 1,2, 1.6 and 1,7 times accordingly. At the 14-th day of acute renal insufﬁciency concentrations of studied macro- and microelements are in the limits of control. At the end of study changes in concentrations of certain macro- and microelements in tissue of kidney are observed. Statistically signiﬁcant decrease of concentration of calcium (1,3 times) and increase of concentration of iron (1,9 times), copper (1,3 times), selenium (1,4 times), lead (2 times) are observed. A tendency to increase concentration of zinc and potassium takes place. Concentration of chlorine is in the limits of control.

At the 3-rd day of the acute renal insufﬁciency concentration of potassium (1,7 times), iron (1,5 times), zinc and chlorine (1,3 times) are increased in regional lymphatic node. Simultaneously concentrations of copper (1,5 times) and selenium (1,6 times) are decreased. Concentrations of the other elements (calcium, lead, manganese) aren’t changed in comparison with control. At the 7-th day increased concentrations of chlorine (1,2 times), potassium (1,9), iron (1,5 times), zinc (1,4 times), lead (1,6 times) and decreased concentrations of copper (1,5 times) and selenium (2,2 times) are statistically signiﬁcant. Concentrations of calcium and manganese are in the limits of control. At the 14-th day of acute renal insufﬁciency concentrations of studied manganese, calcium, iron, zinc and lead are changed in the limits of control. At the end of study (30 days) changes in concentrations of certain macro- and microelements in tissue of lymphatic node are observed. Statistically signiﬁcant decrease of concentration of calcium (1,5 times), copper (1,8 times), zinc (1,3 times), selenium (2,5 times) and increase of concentration of potassium (1,4 times), lead (1,6 times) are observed. Concentration of chlorine and manganese) are in the limits of control.

Exchange of important macro- and microelements is intensiﬁed at the renal insufﬁciency and they accumulate in the tissues of kidney and lymphatic node. Functional synergism takes place in kidney and lymphatic node during the exchange of microelements. It prevents losses of macro- and microelements at the renal insufﬁciency. But compensatory adaptive processes are sufﬁciently expended and accompanied with decreased concentrations of copper and selenium in lymphatic node. More precise deﬁnition of microelement composition of kidney and regional lymphatic node is very important for understanding pathogenesis of acute renal insufﬁciency and gives new possibilities for correction disease based on bioelement exchange.